Translational_Unit

Part:BBa_K3477005:Design

Designed by: Mathilda Johansson   Group: iGEM20_Chalmers-Gothenburg   (2020-10-20)


Gene coding for his-tagged PETase enzyme including BBa_0034 RBS, Bba_J23106 promoter and Bba_0010 te


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 962
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 341
    Illegal XbaI site found at 56
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 341
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal NheI site found at 273
    Illegal SpeI site found at 963
    Illegal PstI site found at 977
    Illegal NotI site found at 7
    Illegal NotI site found at 970
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 341
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 963
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 341
    Illegal XbaI site found at 56
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 341
    Illegal XbaI site found at 56
    Illegal SpeI site found at 963
    Illegal PstI site found at 977
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

Originates from ideonella sakaiensis and was found on Uniprot (https://www.uniprot.org/uniprot/A0A0K8P6T7)

References